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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
21/02/2014 |
Actualizado : |
29/10/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
GAIERO, P.; MAZZELLA, C.; VILARÓ, F.; SPERANZA, P.; DE JONG, H. |
Afiliación : |
PAOLA GAIERO, Department of Plant Biology, Facultad de Agronomía, Universidad de la República, Uruguay; Laboratory of Genetics, Wageningen University, Netherlands; CRISTINA MAZZELLA, Department of Plant Biology, Facultad de Agronomía, Universidad de la República, Uruguay; FRANCISCO LUIS VILARO PAREJA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PABLO SPERANZA, Department of Plant Biology, Facultad de Agronomía, Universidad de la República, Uruguay; HANS DE JONG, Laboratory of Genetics, Wageningen University, Netherlands. |
Título : |
Pairing analysis and in situ Hybridisation reveal autopolyploid-like behaviour in Solanum commersonii × S. tuberosum (potato) interspecific hybrids. |
Fecha de publicación : |
2017 |
Fuente / Imprenta : |
Euphytica, 1 July 2017, Volume 213, Issue 7, Article number 137. OPEN ACCESS. |
ISSN : |
0014-2336 |
DOI : |
10.1007/s10681-017-1922-4 |
Idioma : |
Inglés |
Notas : |
Article history: Received: 29 December 2016 / Accepted: 29 May 2017 / Published online: 14 June 2017. |
Contenido : |
ABSTRACT.
Wild potato relatives are rich sources of desirable traits for introgressive hybridisation into cultivated potato. One of them, Solanum commersonii (2n = 2x = 24, 1EBN, endosperm balance number), is an important species belonging to the potato tertiary genepool. It can be used in potato breeding through bridge crosses and 2n gamete production. Triploid F1 hybrids between S. commersonii (through spontaneous 2n egg formation) and diploid 2EBN Solanum tuberosum Group Phureja were crossed with S. tuberosum Group Tuberosum, resulting in successive backcross 1, 2 and 3 progenies. The main aim of this study was to determine if there are any barriers to homoeologous pairing and recombination in the allotriploid (S. commersonii × potato) hybrids and their backcrosses, and so to predict if S. commersonii chromosomes can be transmitted to the next generation and introgressed into their recipient potato chromosomes. Microscopic observations of spread pollen mother cells suggested no preferential pairing in the triploid hybrids, while chromosome transmission and segregation in further meiotic stages were fairly balanced. Fluorescent in situ hybridisation with BAC probes (BAC FISH) was used to obtain markers to trace the meiotic behaviour of specific chromosome pairs. Moreover, genomic in situ hybridisation (GISH) demonstrated no obvious differences in fluorescence signals between the homoeologues suggesting that repetitive sequences did not diverge much between the parental species. As a consequence, we were not able to trace the course of the S. commersonii chromosomes in the successive introgressive hybridisation backcross generations. Our results strongly point at a high genomic similarity between the homoeologous chromosomes promising high suitability of S. commersonii in introgressive hybridisation breeding of potato.
© 2017, The Author(s). MenosABSTRACT.
Wild potato relatives are rich sources of desirable traits for introgressive hybridisation into cultivated potato. One of them, Solanum commersonii (2n = 2x = 24, 1EBN, endosperm balance number), is an important species belonging to the potato tertiary genepool. It can be used in potato breeding through bridge crosses and 2n gamete production. Triploid F1 hybrids between S. commersonii (through spontaneous 2n egg formation) and diploid 2EBN Solanum tuberosum Group Phureja were crossed with S. tuberosum Group Tuberosum, resulting in successive backcross 1, 2 and 3 progenies. The main aim of this study was to determine if there are any barriers to homoeologous pairing and recombination in the allotriploid (S. commersonii × potato) hybrids and their backcrosses, and so to predict if S. commersonii chromosomes can be transmitted to the next generation and introgressed into their recipient potato chromosomes. Microscopic observations of spread pollen mother cells suggested no preferential pairing in the triploid hybrids, while chromosome transmission and segregation in further meiotic stages were fairly balanced. Fluorescent in situ hybridisation with BAC probes (BAC FISH) was used to obtain markers to trace the meiotic behaviour of specific chromosome pairs. Moreover, genomic in situ hybridisation (GISH) demonstrated no obvious differences in fluorescence signals between the homoeologues suggesting that repetitive sequences did not diverge much between the parental spe... Presentar Todo |
Palabras claves : |
BAC FISH; GISH; Introgressive hybridisation; Pairing behaviour; Potato wild relatives. |
Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
URL : |
https://link.springer.com/content/pdf/10.1007%2Fs10681-017-1922-4.pdf
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Marc : |
LEADER 02814naa a2200265 a 4500 001 1012827 005 2019-10-29 008 2017 bl uuuu u00u1 u #d 022 $a0014-2336 024 7 $a10.1007/s10681-017-1922-4$2DOI 100 1 $aGAIERO, P. 245 $aPairing analysis and in situ Hybridisation reveal autopolyploid-like behaviour in Solanum commersonii × S. tuberosum (potato) interspecific hybrids.$h[electronic resource] 260 $c2017 500 $aArticle history: Received: 29 December 2016 / Accepted: 29 May 2017 / Published online: 14 June 2017. 520 $aABSTRACT. Wild potato relatives are rich sources of desirable traits for introgressive hybridisation into cultivated potato. One of them, Solanum commersonii (2n = 2x = 24, 1EBN, endosperm balance number), is an important species belonging to the potato tertiary genepool. It can be used in potato breeding through bridge crosses and 2n gamete production. Triploid F1 hybrids between S. commersonii (through spontaneous 2n egg formation) and diploid 2EBN Solanum tuberosum Group Phureja were crossed with S. tuberosum Group Tuberosum, resulting in successive backcross 1, 2 and 3 progenies. The main aim of this study was to determine if there are any barriers to homoeologous pairing and recombination in the allotriploid (S. commersonii × potato) hybrids and their backcrosses, and so to predict if S. commersonii chromosomes can be transmitted to the next generation and introgressed into their recipient potato chromosomes. Microscopic observations of spread pollen mother cells suggested no preferential pairing in the triploid hybrids, while chromosome transmission and segregation in further meiotic stages were fairly balanced. Fluorescent in situ hybridisation with BAC probes (BAC FISH) was used to obtain markers to trace the meiotic behaviour of specific chromosome pairs. Moreover, genomic in situ hybridisation (GISH) demonstrated no obvious differences in fluorescence signals between the homoeologues suggesting that repetitive sequences did not diverge much between the parental species. As a consequence, we were not able to trace the course of the S. commersonii chromosomes in the successive introgressive hybridisation backcross generations. Our results strongly point at a high genomic similarity between the homoeologous chromosomes promising high suitability of S. commersonii in introgressive hybridisation breeding of potato. © 2017, The Author(s). 653 $aBAC FISH 653 $aGISH 653 $aIntrogressive hybridisation 653 $aPairing behaviour 653 $aPotato wild relatives 700 1 $aMAZZELLA, C. 700 1 $aVILARÓ, F. 700 1 $aSPERANZA, P. 700 1 $aDE JONG, H. 773 $tEuphytica, 1 July 2017, Volume 213, Issue 7, Article number 137. OPEN ACCESS.
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
02/01/2017 |
Actualizado : |
02/01/2017 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
FIERRO, S.; VIÑOLES, C.; OLIVERA-MUZANTE, J. |
Afiliación : |
S. FIERRO, SUL (Secretariado Uruguayo de la Lana).; CAROLINA VIÑOLES GIL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; J. OLIVERA-MUZANTE, Universidad de la República (UdelaR)/ Centro Universitario Regional Noroeste, Salto (CENUR). |
Título : |
Long term prostaglandin based-protocols improve the reproductive performance after timed artificial insemination in sheep. |
Fecha de publicación : |
2017 |
Fuente / Imprenta : |
Theriogenology, 2017, v. 90, p. 109-113. |
DOI : |
10.1016/j.theriogenology.2016.11.031 |
Idioma : |
Inglés |
Notas : |
Article history: Received 25 April 2016; Received in revised form; 28 September 2016; Accepted 30 November 2016; Available online 2 December 2016 |
Contenido : |
ABSTRACT.
The aim of the experiment was to evaluate the reproductive performance of ewes synchronized with two doses of prostaglandin F2a (PG) at different intervals and inseminated at a fixed time. During the breeding season (April to June), 370 multiparous Corriedale ewes were assigned to five groups according to body condition score and body weight, and synchronized with two doses of PG administered 7, 10, 12, 14 or 16 days apart (groups PG7, PG10, PG12, PG14 or PG16; n ¼ 73, 76, 74, 72, 75; respectively). Cervical timed artificial insemination (Day 0) was performed at 48 ± 1.0 h (group PG7) or 56 ± 1.0 h (groups PG10, PG12, PG14 and PG16) after the second PG injection, with diluted fresh semen pooled from six adult rams. The percentage of ovulating ewes after the second PG injection and the ovulation rate (number of corpus luteum/ovulating ewes) were assessed on Day 10 by trans-rectal ultrasonography. The rate of non return to service (ewes not returning to service/inseminated ewes 100; NRR-21) was evaluated using painted vasectomized rams. Pregnancy rate (pregnant ewes/inseminated ewes 100) and prolificacy (foetuses/pregnant ewes) were determined on Day 60 by trans-abdominal ultrasonography. Higher NRR-21 and pregnancy rates was observed in groups PG12 (46.0%, 46.0%), PG14 (59.7%, 56.9%) and PG16 (58.7%, 56.0%) compared to PG7 (30.1%, 28.8%) and PG10 (30.3%, 30.3%; respectively P < 0.05). No significant differences were observed in the percentage of ovulating ewes, ovulation rate and prolificacy among groups (P > 0.05). Under the condition of this trial, 12, 14 or 16 days interval between PG injections enhances the pregnancy rate of ewes at cervical timed artificial insemination with fresh semen.
© 2016 Elsevier Inc. All rights reserved. MenosABSTRACT.
The aim of the experiment was to evaluate the reproductive performance of ewes synchronized with two doses of prostaglandin F2a (PG) at different intervals and inseminated at a fixed time. During the breeding season (April to June), 370 multiparous Corriedale ewes were assigned to five groups according to body condition score and body weight, and synchronized with two doses of PG administered 7, 10, 12, 14 or 16 days apart (groups PG7, PG10, PG12, PG14 or PG16; n ¼ 73, 76, 74, 72, 75; respectively). Cervical timed artificial insemination (Day 0) was performed at 48 ± 1.0 h (group PG7) or 56 ± 1.0 h (groups PG10, PG12, PG14 and PG16) after the second PG injection, with diluted fresh semen pooled from six adult rams. The percentage of ovulating ewes after the second PG injection and the ovulation rate (number of corpus luteum/ovulating ewes) were assessed on Day 10 by trans-rectal ultrasonography. The rate of non return to service (ewes not returning to service/inseminated ewes 100; NRR-21) was evaluated using painted vasectomized rams. Pregnancy rate (pregnant ewes/inseminated ewes 100) and prolificacy (foetuses/pregnant ewes) were determined on Day 60 by trans-abdominal ultrasonography. Higher NRR-21 and pregnancy rates was observed in groups PG12 (46.0%, 46.0%), PG14 (59.7%, 56.9%) and PG16 (58.7%, 56.0%) compared to PG7 (30.1%, 28.8%) and PG10 (30.3%, 30.3%; respectively P < 0.05). No significant differences were observed in the percentage of ovulating ewes, o... Presentar Todo |
Palabras claves : |
EWE; FERTILITY; OESTRUS SYNCHRONIZATION; OVULATION RATE; PROTAGLANDIN; TIMED ARTIFICAL INSEMINATION. |
Thesagro : |
OVEJA; OVINOS. |
Asunto categoría : |
-- |
Marc : |
LEADER 02707naa a2200265 a 4500 001 1056404 005 2017-01-02 008 2017 bl uuuu u00u1 u #d 024 7 $a10.1016/j.theriogenology.2016.11.031$2DOI 100 1 $aFIERRO, S. 245 $aLong term prostaglandin based-protocols improve the reproductive performance after timed artificial insemination in sheep.$h[electronic resource] 260 $c2017 500 $aArticle history: Received 25 April 2016; Received in revised form; 28 September 2016; Accepted 30 November 2016; Available online 2 December 2016 520 $aABSTRACT. The aim of the experiment was to evaluate the reproductive performance of ewes synchronized with two doses of prostaglandin F2a (PG) at different intervals and inseminated at a fixed time. During the breeding season (April to June), 370 multiparous Corriedale ewes were assigned to five groups according to body condition score and body weight, and synchronized with two doses of PG administered 7, 10, 12, 14 or 16 days apart (groups PG7, PG10, PG12, PG14 or PG16; n ¼ 73, 76, 74, 72, 75; respectively). Cervical timed artificial insemination (Day 0) was performed at 48 ± 1.0 h (group PG7) or 56 ± 1.0 h (groups PG10, PG12, PG14 and PG16) after the second PG injection, with diluted fresh semen pooled from six adult rams. The percentage of ovulating ewes after the second PG injection and the ovulation rate (number of corpus luteum/ovulating ewes) were assessed on Day 10 by trans-rectal ultrasonography. The rate of non return to service (ewes not returning to service/inseminated ewes 100; NRR-21) was evaluated using painted vasectomized rams. Pregnancy rate (pregnant ewes/inseminated ewes 100) and prolificacy (foetuses/pregnant ewes) were determined on Day 60 by trans-abdominal ultrasonography. Higher NRR-21 and pregnancy rates was observed in groups PG12 (46.0%, 46.0%), PG14 (59.7%, 56.9%) and PG16 (58.7%, 56.0%) compared to PG7 (30.1%, 28.8%) and PG10 (30.3%, 30.3%; respectively P < 0.05). No significant differences were observed in the percentage of ovulating ewes, ovulation rate and prolificacy among groups (P > 0.05). Under the condition of this trial, 12, 14 or 16 days interval between PG injections enhances the pregnancy rate of ewes at cervical timed artificial insemination with fresh semen. © 2016 Elsevier Inc. All rights reserved. 650 $aOVEJA 650 $aOVINOS 653 $aEWE 653 $aFERTILITY 653 $aOESTRUS SYNCHRONIZATION 653 $aOVULATION RATE 653 $aPROTAGLANDIN 653 $aTIMED ARTIFICAL INSEMINATION 700 1 $aVIÑOLES, C. 700 1 $aOLIVERA-MUZANTE, J. 773 $tTheriogenology, 2017$gv. 90, p. 109-113.
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